ABSTRACT
Objective:To evaluate the clinical efficacy of endoscope assisted supraclavicular thyroidectomy and lymph node dissection through the sternocleidomastoid muscular approach (ELDS) .Methods:Clinical data of 40 patients undergoing ELDS and 40 patients who had open surgery (open group) by same team admitted to Ningbo Medical Center Lihuili Hospital from Jan. 2021 to Jan. 2022 were retrospectively analyzed. The intraoperative and postoperative outcomes and follow-up were observed situation. The intraoperative situation, the number of lymph nodes dissected, postoperative outcomes and scar healing satisfaction were observed. SPSS 20.0 software was employed to carry out statistical analysis, the measurement date were compared with t-test, and the counting date were analyzed by χ2 test. Results:There was no significant differences in the two groups with respect to the number of lymph nodes dissected (ELDS group: 30.5±9.8, open group: 29.9±9.0) . The surgical injury in ELDS group were significantly smaller than those in open group, and the operation time was significantly higher in open group (ELDS group: 95.2±12.0min, open group: 82.3±13.9min, P<0.05) . In postoperative follow-up, there were significant differences between the two groups in swallowing impairment, anterior cervical pressure and scar satisfaction (ELDS group: 4.45±1.82, open group: 6.03±1.47, P<0.05) , and the lateral approach group was superior to open group, but there were no significant differences in parathyroidism or recurrent laryngeal nerve injury ( P>0.05) . Conclusions:ELDS has the advantages of good cosmetic effect, less postoperative anterior cervical discomfort, less postoperative complications, and good lymph node dissection effect. The operation is safe and feasible, and has obvious advantages over traditional operation.
ABSTRACT
@#Prostate cancer is one of the most common cancers in adult men. Heat shock proteins (HSPs),as molecular chaperones widely involved in the pathogenesis,diagnosis,treatment and prognosis of various cancers,play crucial biological functions in prostate cancer and it can be considered as valuable biomarkers for cancer therapy, such as prostate-specific membrane antigen. As a member of the heat shock protein family, HSP27 is related to prostate cancer castration resistance,and its expression can promote tumor resistance,invasion and bone metastasis,making prostate cancer more invulnerable to treatments. Therefore,targeting HSP27 in prostate cancer can be perceived as one promising cancer treatment strategy. This article reviews the structure and function of HSP27,and its potential role on castration resistance and targeted therapy in order to provide a new theoretical basis for the clinical treatment of prostate cancer.
ABSTRACT
Bronchopulmonary dysplasia (BPD) remains a common problem in preterm infants and affects the prognosis of these infants. Apart from playing a role in bone metabolism, vitamin D is also involved in regulation of gene expression, immunity, inflammation responses and lung development and restoration, which happens to coincide with the pathological process of BPD, suggesting its potential role in the pathogenesis of BPD. In addition, preterm infants are at high risk of vitamin D deficiency, hence more in-depth research on the association between vitamin D deficiency and BPD in preterm infants is needed. In recent years, some animal experiments had shown evidence of the benefits from vitamin D against BPD, but more clinical research is required. Vitamin D supplementation in early life may be a new approach to prevent and treat BPD.
ABSTRACT
Bronchopulmonary dysplasia (BPD) remains a common problem in preterm infants and affects the prognosis of these infants. Apart from playing a role in bone metabolism, vitamin D is also involved in regulation of gene expression, immunity, inflammation responses and lung development and restoration, which happens to coincide with the pathological process of BPD, suggesting its potential role in the pathogenesis of BPD. In addition, preterm infants are at high risk of vitamin D deficiency, hence more in-depth research on the association between vitamin D deficiency and BPD in preterm infants is needed. In recent years, some animal experiments had shown evidence of the benefits from vitamin D against BPD, but more clinical research is required. Vitamin D supplementation in early life may be a new approach to prevent and treat BPD.
ABSTRACT
Objectives Exploring the association between depression/anxiety and mental stress-induced myocardial ischemia (MSIMI) in patients with stable coronary artery disease (CAD). Methods A total of 178 subjects was enrolled according to the inclusion and exclusion criterion with 88 men and 90 women at age of (54±12)years. The subjects were divided into four groups including CAD with depression/anxiety, CAD without depression/anxiety, depression/anxiety without CAD, and control group based on the state of coronary artery, the scores of Patient Health Questionnaire 9-item (PHQ-9) and Generalized Anxiety Disorder 7-item(GAD-7). MSIMI was diagnosed by echocardiography. Data were analyzed by SPSS19.0. Results The incidence of MSIMI in all CAD patients was 17.24%. Within each group, 35.00% patients were MSIMI in CAD with depression/anxiety, 2.13% were in CAD without depression/anxiety, 14.29% were in depression/anxiety without CAD, and 2.38% were in control group. The risks of MSIMI in depression/anxiety without CAD and with CAD groups were 6.83 (P>0.05) and 22.08 times (P<0.05) higher than that in control group, respectively. Logistic regression showed that a 1-point increment in the GAD-7 score, but not PHQ-9 score [ OR=0.95, 95% CI (0.77-1.17), P=0.63], was associated with 1.22-fold increase in the likehood of MSIMI [95% CI (1.07-1.38), P=0.00]. Conclusions The MSIMI rate is much higher in patients with CAD comorbid depression/ anxiety compared with CAD without depression/anxiety. Anxiety, but not depression, is an independent risk factor of MSIMI in CAD patients.
ABSTRACT
Objective To study the value of new 16S rRNA gene chip in pathogen detection of neonatal sepsis.Method Newborns with suspected sepsis hospitalized in Peking University Shenzhen Hospital from January 2015 to December 2017 were chosen as the subjects.Blood culture and gene chip were both used to detect the pathogens of these infants.The positive rate,the detection time,and the blood volume needed for detection were compared between the two methods.Result A total of 306 cases of suspected neonatal sepsis were included in the study.Among them,34 (11.1％) were positive for blood culture and 54 (17.6％) were positive for gene chip.98 cases were diagnosed as neonatal sepsis,34 (34.7％) were positive for blood culture,and 52 (53.1％) were positive for gene chip.The positive rate of gene chip was higher than that of blood culture (P ＜ 0.05).For the 5 common pathogens of neonatal sepsis,the positive rate of gene chip was higher than that of blood culture.Time to positivity (TTP) and pathogen identification time of blood culture were (14.6 ± 5.5) h and (72.9 ± 19.0) h,respectively.TTP and pathogen identification time of gene chip were both 3 h.The detection time of gene chip was significantly less than that of blood culture (P ＜ 0.001).The blood volume needed for detection of blood culture and gene chip was 1 ～ 2 ml and 0.5 ml.Gene chip needs less blood volume than blood culture.Conclusion Compared with the traditional blood culture,gene chip can quickly detect the pathogens in the blood with higher positive rate and less blood volume.Gene chip is of great value in the diagnosis of neonatal sepsis.
ABSTRACT
The high morbidity and compliments of patent ductus arteriosus (PDA) in preterm infants are reported,but the diagnostic and prognostic appraisal of PDA is still debatable.The B-type natriuretic peptide (BNP) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) are found efficient in diagnosis of hemodynamically significant patent ductus arteriosus (hsPDA) in preterm infants,and also helpful in therapy decisions and prediction of treatment response.However,the type of assay and cut-off values have not yet reached a consent,so that further researches are needed.
ABSTRACT
Objective To investigate the effect of ultrashort wave therapy on edema and inflammatory reaction after spinal cord injury (SCI) in rats. Methods 56 Sprague-Dawley rats were divided into sham-operated group (n=8), model group (n=24) and ultrashort wave group (n=24). The model was established with Allen's method. The sham-operated group was exposed endorhachis without hit. The ultra-short wave group was exposed to ultrashort wave radiation 7 minutes, once a day, 24 hours after modeling until the animals were sacrificed. Locomotors functional recovery was assessed every week post operation period by BBB score. Immunohistochemical staining was per-formed to observe the expression of the aquaporin-4(AQP-4) and ED-1. Results The BBB scores increased in the model group and the ultra-short wave group after treatment, and was higher in the ultrashort wave group than in the model group from 1 week after treatment (t>3.368, P3.156, t>4.466, P<0.05). Conclu-sion Ultrashort wave therapy can alleviate the edema after SCI, reduce the activation and infiltration of inflammatory cells, and promote the recovery of neurological function.
ABSTRACT
Objective To investigate the clinical value of of procalcitonin detection in neonatal necrotizing enterocolitis (NEC).Methods Sixty cases eligible NEC infants were randomly divided into observation group and control group(n=30 each group).All the infants were divided into Ⅰ grade group(19cases),Ⅱ-Ⅲ grade group(41 cases) according to NEC classification.All infants received PCT detection after NEC diagnosis.The observation group received antibiotic treatment base on the serum PCT levels.The control group received antibiotic treatment according to treatment routine and physician's clinical experience.Results PCT increased in 28 (46.7％) of the 60 NEC infants.In the Ⅰ grade group and Ⅱ-m grade group,the proportion of PCT increased cases were 26.3％,61.3％ respectively,and the PCTmax in PCT positive cases were (0.81±0.25) μg/L,(1.76±0.89) μg/L respectively,the differences were signifiacnt(x2 =4.627,t=-2.608,P＜0.05).The time of antibiotic usage in observation group was significantly less than that in control group ((4.9±3.0) d vs.(8.6±2.5) d,t=-3.645,P＜0.01).The fasting time and the cure time of the two groups had no significant difference (P＞ 0.05).Conclusion PCT detection can help to understand the cause and severity of NEC.Using antibiotics according to PCT levels can reduce the time of antibiotic usage without affecting the effect of the NEC treatment.
ABSTRACT
Objective To investigate the effects of bone marrow stromal cells(BMSCs)transplantation combined with low dose ultrashort wave (USW)radiation on functional recovery and the expression of glial fibrillary acidic protein(GFAP)and ED?1 after spinal cord injury(SCI)in rats,and further discuss its action mechanism. Methods Female Sprague?Dawley rats(n=30)were randomly divided into 5 groups:sham?oper?ated,as well as control,USW,BMSCs,and USW+BMSCs that were subjected to spinal cord injury(SCI). Basso?Beattie?Bresnahan(BBB)tests were carried out before the operation and at 1 d,1 week,2 weeks,3 weeks,4 weeks after SCI. 4 weeks later,animals were sacrificed and tissues were collected to make paraffin section. Immunohistochemical staining was performed to observe the expression of GFAP and ED?1. Results 4 weeks after SCI,BBB scores were significantly higher in the USW and USW+BMSCs groups than in the control group(both P<0.001). No signifi?cant difference was observed between the BMSCs group and control group. On the expression of GFAP ,only USW+BMSCs group showed signifi?cantly decreased compared with the control group(P<0.05). All treatment groups exhibited lower ED?1 expression than the control group(all P<0.05). Conclusion Our results indicate that USW radiation alone can obviously improve neural functional recovery after SCI. The USW radi?ation and BMSCs transplantation treatment can reduce inflammation ,and USW radiation is more effective. The combination therapy did not show a synergistic action on promoting functional recovery ,but do have an effect on reducing the inflammatory response and glial scar formation.
ABSTRACT
Objective To discuss the risk factors for cardiac complications in infants of diabetic mothers (IDMs).Methods One hundred and seventy-one out of 228 IDMs hospitalized in Department of Pediatrics,Shenzhen Hospital of Peking University from Jan.2011 to Apr.2014,were involved,among them,there were 171 cases who had the complete mother's data during pregnancy,and they were received B ultrasonography whin 1 week after birth.All the infants were divided into 2 groups according to echocardiography within 1 week after birth:the observation group (the patients had cardiac complications) and the control group (the patients had normal heart).Conditions of IDMs and their mothers between the 2 groups were recorded and compared.Results In the 171 IDMs,there were 69 cases(38male and 31 female) in the observation group and 102 cases(59 males and 43 females) in the control group.There were 24 preterm infants,16 large for gestational age(LGA) infants in the observation group,while 47 preterm infants and 15 LGA in the control group,and which had no statistical difference between the both groups(x2 =0.129,2.163,1.995 ; all P ＞ 0.05).In the observation group,there were 7 (4.1％) newborns with hypoglycemia,24 (14.0％) women with advanced maternal age,24(14.0％) women with other gestational complications,19(11.1％) women having good control of their glucose serum levels,and 9(5.3％) women with impaired glucose tolerance.All the mentioned factors had statistical differences when compared with the control group[1 (0.6％) case,19 (11.1％) cases,15 (8.8％) cases,80(66.8％) cases,26(15.2％) cases] (x2 =7.752,5.707,9.423,43.735,3.917 ;all P ＜ 0.05,0.01).Logistic regression showed that women with other gestational complications as risk factors(OR =3.29,95％ CI 1.248-8.671),while having a good control of the glucose serum levels acted as a protectable factor(OR =0.098,95％ CI 0.045-0.217).Conclusions IDMs are prone to have cardiac complications if their mothers couldn't control the glucose serum levels during pregnancy,with other gestational complication and advanced maternal age and with more serious diabetes.
ABSTRACT
@#ObjectiveTo discuss the relationship between sit-to-stand velocity and dynamic and static balance respectively in stroke patients.Methods22 stroke patients were measured their time for Five Times Sit to Stand Test (FTSTS scores) and the static balance performance as well as swaying of center of gravity in lateral directions was recorded with GS-10A. In addition, the distances of functional reach test (FRT) when sitting were measured.ResultsBy using Pearson's correlation coefficient, FTSTS scores showed the moderate correlation with hemiparetic side, ENV. Area and distance of sitting FRT (r=-0.512, P<0.05；r=0.632, P<0.05；r=-0.662, P<0.01). Linear regression model, in terms of balance ability, showed the main factors associated with the sit to stand velocity were the distance of sitting FRT and ENV.Area. ConclusionENV.Area and distance of sitting FRT are the main factors associated with sit to stand velocity and both static and dynamic balance can predict sit to stand velocity.
ABSTRACT
Objective To discuss the clinicd effect of using nebulised budesonide to treat neonatal laryneal edema caused by mechanical ventilation.Methods 43 newborns with mechanical ventilation were included in this study and divided into two groups:The Observational group(23 newborns)were treated with nebulised budesonide right before excubation once and three times after that,and the control group(20 newborns)were treated with dexamethasone once right before excubation and nebulised adrenine after that for three times.Comparisons were made between two groups about dismissing time of wheeze or hoarseness,tachypnea,PaO2 and PaCO2.Results Observational group:dismissing time of wheeze or hoarseness was(10.26±4.01)hours,dismissing time of tachypnea was(1.63±0.55)days,which was shorter than that of control group[(14.20±5.58)hours/(2.05±0.58)days],P＜0.05).PaO2 and PaCO2 of two groups had no statistical difference(P＞0.05).Conclusion Nebulised budesonide was effective in treatment neonatal laryneal edema caused by mechanical ventilation,using.
ABSTRACT
Objective To evaluate the efficacy of blue-green light phototherapy for jaundice in preterm newborns.Methods Total 124 preterm newborns with birth-weight below 2000 g were included in this study.Infants in group A (gestation age ≤32 weeks or/and instability,n =72) were treated with bluegreen light (group A1 ) or with single side blue light (group A2) randomly.Infants with gestation age ＞32weeks or/and stability (n =52) were also treated with blue-green light (group B1 ) or with single side blue light ( group B2) randomly.Peak levels of serum bilirubin ( PSB),the withdrawal time of jaundice and the duration of phototherapy were compared between groups.Results Group A:PSB of groupAl was lower than that of groupA2 [ (219 ± 31 ) μmol/L vs.( 257 ± 39 ) μ mol/L,P ＜ 0.01 ].The withdrawal time of jaundice group Al was earlier than that of group A2 [ ( 10.3 ±3.0) d vs.( 16.2 ±4.2) d,P ＜0.01 ].The duration of phototherapy of groupA1 was shorter than that of group A2 [ (87 ±34) h vs.(23 ±49) h,P ＜0.01 ].Group B:There were no differences in PSB and withdrawal time of jaundice between two subgroups ( P ＞0.05).The duration of phototherapy of groupB1 was shorter than that of group B2 [ (59 ±23) h vs.(73 ±74) h,P ＜ 0.05 ].Conclusion Blue-green light phototherapy is more effective than single side of blue light in treatment of jaundice in preterm newborns.
ABSTRACT
Objective To find out the clinical characteristics and morbility factors of intravascular catheterrelated bloodstream infection(CRBSI). Methods Totally 21 patients who had CRBSI in neonatal intensive care unit were investigated retrospectively. Results The distribution of CRBSI was higher in very low birthweight preterm infants, gestational age among 28 ～34week, whose intravascular catheter remaining time were obove three weeks. Principal clinical presentation of CRBSI were poor feeding, unaocountable tachycardia, temperature instability, stressed hyperglycemia,refractoriness metabolic acidosis. The most common pathogens were coagulase-negative staphylococci (35.7%), Klebsiella pneumonia, bacilli ( 11.9% ) Staphylococcus aureus (9.5 % ), Pseudomonas aeruginosa( 7. 1% )and Enterobacter cloacae(7.1% ). Conclusions The clinical manifestations of CRBSI were concealment,and reducing the time of inserted central catherization and total parenteral nutrition, strengthening the nutrition of body would provide effective prevention of CRBSI.
ABSTRACT
AIM: To establish HPLC for the determination of icariin and adenosine in Cordyceps Vigorine Tablet. METHODS:MetaChem Polaris ODS C18-A column(4.6 mm × 250 mm, 5 μm) was adopted; acetonitrile-water was the mobile phase; detection wavelength was set at 272 nm for icariin and at 260 nm for adenosine; column linearity within the range of 0. 374-2. 246 μg for icariin and 0. 348-1. 392 μg for adenosine, respectively. The average recovery and RSD were found to be (99.4 ± 1.84) % for icariin ( n = 6), (99.63 ± 1.29 ) % for adenosine,respectively. CONCLUSION: The method is convenient, fast, reliable to operate and suitable for the quality control of Cordyceps Vigorine Tablet.
ABSTRACT
Objective To study a fingerprint of raw material of Rhizoma Polygoni Cuspidati from(various) habitats by RP-HPLC. Methods Kromasil C_(18) column(150 mm?4.6 mm,5 ?m) was used,with mixtures of acetonitrile and water as mobile phase in a gradient mode.The flow rate was 1 mL/min and wavelength was 303 nm.Results According to the optimum chromatographic conditions,a good fingerprint of Rhizoma Polygoni Cuspidati has been described.Conclusion The method is simple and accurate with good reproducibility.It may be practical value for the quality control of sample for Rhizoma Polygoni Cuspidati from various habitats.